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. Author manuscript; available in PMC: 2023 Apr 20.
Published in final edited form as: J Med Chem. 2021 Dec 15;64(24):18025–18053. doi: 10.1021/acs.jmedchem.1c01382

Figure 10.

Figure 10.

Apoptotic effect of 44e on TNBC cells. (A, B) MDA-MB-231 and MDA-MB-468 cells were treated with indicated concentrations of 44e for 24 h. Apoptosis ratios were determined by flow cytometry analysis of Annexin-V/PI double staining. A representative image and quantification of apoptosis are presented. (C, D) Cells were treated with 44e at different concentrations for 24 h. The levels of apoptosis-related proteins, including Bcl-2, Bax, caspase-3, and cleaved caspase-3, were analyzed by Western blotting. GAPDH was used as load control. Error bar shows SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with the control groups.