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. Author manuscript; available in PMC: 2023 Apr 20.
Published in final edited form as: J Med Chem. 2021 Dec 15;64(24):18025–18053. doi: 10.1021/acs.jmedchem.1c01382

Figure 12.

Figure 12.

Mechanism of autophagy in 44e-induced cell death in TNBC cells. (A, B) MDA-MB-231 and MDA-MB-468 cell viability was measured by MTT assay. Cells were treated with 44e at 5 μM or combined with 3-MA at 1 mM treated for 24 h. 3-MA was added 1 h before treatment with 44e. (C) MDA-MB-231 and MDA-MB-468 cell apoptosis ratios were analyzed by flow cytometry with Annexin-V/PI double staining. Cells were treated with 44e at 5 μM or combined with 3-MA at 1 mM for 24 h. 3-MA was added 1 h before treatment with 44e. (D, E) Colony formation assay in MDA-MB-231 and MDA-MB-468 cells. Cells treated with 44e at 5 μM or combined with 3-MA at 1 mM. Error bar shows SD; *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control groups.