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. 2023 Apr 3;2(4):pgad115. doi: 10.1093/pnasnexus/pgad115

Fig. 5.

Fig. 5.

JG-98 selectively decreases protein levels of mitochondrial ribosomal subunits. A) GO Cell Component enrichment analysis of most strongly down-regulated proteins in 22Rv1 cells after 4 h treatment with 5 µM JG-98. B) Table of mitochondrial ribosomal subunits (MRPs) and assembly factors down-regulated (≥ 25%) due to JG-98 treatment. C) Western blot analysis of select MRPs from 22Rv1 whole cell extract following treatment of intact cells with increasing concentrations of JG-98 (0, 1, 2.5, 5, 7.5, or 10 µM) for 6 h (left) or 24 h (right). D) Mitochondrial protein translation assay using mitochondria isolated from 22Rv1 cells treated with 125 nM or 250 nM JG-98 or inactive analog (JG-258) for 24 h. Detection of HPG incorporation into newly synthesized mitochondrial proteins (top panel); western blot detection of VDAC (bottom panel, load control).