Skip to main content
. 2023 Apr 3;2(4):pgad115. doi: 10.1093/pnasnexus/pgad115

Fig. 6.

Fig. 6.

JG-98 targets mitochondrial HSP70 (HSPA9) to impact MRP stability and aggregation. A) Western blot of MRP protein solubility/insolubility in 22Rv1 cells treated with 5 µM JG-98 for 0, 1, 2, 3, 4, or 6 h. B) Western blot analysis of MRP solubility/insolubility in 22Rv1 cells pretreated with 10 µg/mL cycloheximide (CHX) or 10 µM FCCP +/− 5 µM JG-98 (3 h). C) FCCP impact on JG-98 induced MRP aggregation in 22Rv1 cells treated for 3 h. D) Anti-FLAG immunoprecipitation (IP) from HEK293 cells transfected with empty vector (EV) or HSPA9-FLAG. Cells were treated for 1, 3, or 6 h with 0, 1, 2.5, or 5 µM JG-98 prior to lysis and IP. GRPEL1 is a mitochondrial BAG/NEF; LONP1, CLPP, and AFG3L2 are mitochondrial matrix proteases. E) Impact of LONP1-, CLPP-, or AFG3L2-KD on JG-98-induced aggregation of MRP subunits (3 h treatment).