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. 2023 Mar 30;9(4):661–672. doi: 10.1038/s41477-023-01377-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — Altered photosystem protein abundance in sig5-3 mutant. (A, B) Automated semi-quantitative immunoassay comparing (A) PSII D2 and (B) PSAC protein abundance between wild type (Col-0) and sig5-3 under control temperature conditions (20 °C) and after freezing at -8 °C for 6 h (FRZ). Analysis shows two independent experiments, each containing triplicate immunodetection analyses. (C) Coomassie blue-stained SDS-PAGE separation of 0.5 mg/mL total protein from leaf protein extracts, run as a single example to demonstrate consistent protein input into the automated semi-quantitative immunoassay when samples were prepared identically for immunodetection.