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. 2023 Mar 30;9(4):661–672. doi: 10.1038/s41477-023-01377-1

Extended Data Fig. 6. RbcL protein abundance is unaltered by sig5-3 mutant and freezing.

Extended Data Fig. 6

RbcL protein abundance is unaltered by sig5-3 mutant and freezing. (A) Automated semi-quantitative immunoassay comparing RbcL protein abundance between wild type (Col-0) and sig5-3 under control temperature conditions (20 °C) and after freezing at -8 °C for 6 h (FRZ). Analysis shows two independent experiments, each containing triplicate immunodetection analyses. (B) Comparison of RbcL protein abundance from (A, B). Circles on plots indicate result from each independent experiment (two independent repeats, each with three technical replicates). (C, D) Ratio of (C) mean PSII D2 abundance and (D) mean PSAC abundance to mean RbcL abundance, calculated from the protein abundance data in Fig. 4D, F and Extended Data Fig. 6B.