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. 2023 Mar 30;9(4):661–672. doi: 10.1038/s41477-023-01377-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — Light spectra and RT-qPCR controls. (A, B) Comparison of spectra in Panasonic MLR-352 growth chambers set to (A) control and (B) cold temperature conditions. (C) Spectrum of Conviron growth chambers used to propagate mature plants. (D) Comparison of response of SIG5 to cold, at two different timepoints, in two background lines, and in the hy5 hyh double mutant. The transcript dynamics are broadly similar when each of ACT2, EF-1A and UBQ10 was used as a reference gene. *** = p < 0.001; ** = p < 0.01, * = p < 0.05 and n.s = not significant in unpaired two-sided t-tests, n = 3 biological replicates shown as mean + /- s.e.m. p values: Col-0 ACT2 (ZT25 0.002; ZT37 0.000), Col-0 EF-1A (ZT25 0.011; ZT37 0.000), Col-0 UBQ10 (ZT25 0.002; ZT37 0.014), Ws EF-1A (ZT25 0.000; ZT37 0.239), Ws UBQ10 (ZT25 0.006; ZT37 0.239), hy5 hyh EF-1A (ZT25 0.534; ZT37 0.57), hy5 hyh UBQ10 (ZT25 0.432; ZT37 0.048).