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. 2023 Mar 30;9(4):661–672. doi: 10.1038/s41477-023-01377-1

Fig. 2. Circadian gating of the responses to cold of SIG5 and chloroplast psbD BLRP, and the involvement of HY5 and HYH.

Fig. 2

a,b, Circadian gating of the response to cold of SIG5 (a) and psbD BLRP (b) transcripts in the Col-0 wild type and sig5-3 mutant. c–f, Circadian gating of the response to cold of SIG5 (c–e) and psbD BLRP (f–h) in the hy5, hyh or hy5 hyh double mutant. Cold treatments comprised 3 h at 4 °C for SIG5 transcript levels, and 5 h at 4 °C for psbD BLRP. Each short cold treatment was applied to a separate batch of seedlings. The x axis indicates the time at which the cold treatment commenced. Grey shading on graphs indicates subjective night, under constant light conditions. Solid and broken lines indicate control (19 °C) and cold (4 °C) treatments, respectively. Wild-type data (black lines) are duplicated across c–e and f–h for visual clarity. Experiments used 11-day-old seedlings. Data represent mean ± s.e.m. of three independent biological replicates. Statistical information above graphs compares the transcript levels in the wild type and mutant under control temperature conditions (grey text) and in response to cold (blue text) at each time point. ***P < 0.001; **P < 0.01; *P < 0.05; NS, not significant in unpaired two-sided t-tests. Exact P values are given in Supplementary Data 1.