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. 2023 Mar 30;9(4):661–672. doi: 10.1038/s41477-023-01377-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — SIG5 transcript accumulation during cold treatments of up to 24 h. (A) Relative abundance of SIG5 transcripts in Col-0 wild type and sig5-3 plants after cold treatment (4 °C) for durations ranging from 30 mins to 24 hours. (B) Abundance of SIG5 transcripts in wild type (Col-0) and two athb17 mutants after 3 h at 19 °C or 4 °C. Plants were under constant light conditions and given a 3 h cold treatment 1 h after subjective dawn. Experiments used 11-day old seedlings. Data are mean + /- s.e.m. (n = 3 independent biological replicates). Statistical comparisons are of (A) transcript abundance between Col-0 19 °C and Col-0 4 °C and (B) transcript abundance between control temperature and cold-treated plants, and between genotypes. Data analysed by (A) one-way ANOVA and (B) two-way ANOVA, both followed by one-sided post-hoc Tukey test, where *** = p < 0.001; ** = p < 0.01, * = p < 0.05 and n.s = not significant. p-values (A) 19 °C vs 4 °C for Col-0 at 0 h (p = 1.0000), 0.5 h (p = 0.9277), 1 h (p = 1.0000), 3 h (p = 0.1867), 6 h (p < 0.0001), 9 h (p < 0.0001), 12 h (p = 0.0001), 24 h (p < 0.0001); (B) 19 °C vs 4 °C for Col-0 (p = 0.0000425), athb17-1 (p = 0.0008523); athb17-2 (p = 0.0010056); Col-0 vs athb17-1 at 4 °C (p = 0.1958629); Col-0 vs athb17-2 at 4 °C (p = 0.0918899).