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. 2023 Mar 30;9(4):661–672. doi: 10.1038/s41477-023-01377-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — Accumulation of (A) SIG5 and (B) psbD BLRP transcripts in the wild type (Ws), hy5, hyh and hy5 hyh double mutant. Experiments used 11-day old seedlings. All data represent means + /- s.e.m. of three independent biological replicates. Statistical comparison of each mutant with the wild type, at each timepoint, is provided above time-series plots, where *** = p < 0.001; ** = p < 0.01, * = p < 0.05 and n.s = not significant (one-way ANOVA followed by one-sided post-hoc Tukey test; 3 biological replicates; p values for panels A and B in Supplementary Data 1). (C) Quantification of luciferase bioluminescence after transient expression of SIG5::LUCIFERASE in wild type, hy5, hyh and hy5 hyh mutants, at two timepoints, using particle bombardment (n = 8 replicates per condition and genotype, except for Ws at ZT26 where n = 6; mean + /- s.e.m.). (D) Response of HY5 and HYH transcripts to a treatment of 3 h at 4 °C, given at two different timepoints, under free running conditions. Transcript levels normalized to ACT2 and confirmed with two other reference transcripts (not shown), 3 biological replicates show as mean + /- s.e.m; *** = p < 0.001; ** = p < 0.01, * = p < 0.05 and n.s = not significant in unpaired two-sided t-tests. p values for (D) are Col-0 HY5 (ZT25 0.002; ZT37 0.000), Ws HY5 (ZT25 0.001; ZT37 0.006), Col-0 HYH (ZT25 0.003; ZT37 0.000), Ws HYH (ZT25 0.000; ZT37 0.098).