Fig. 2.

MiR-155-5p overexpression inhibited nuclear PTEN expression by targeting Ndfip1. MiR-155-5p mimic (50 nM) or miR-155-5p mimic (100 nM) was used to transfect astrocytes for 24 h. A The predicted miR-155-5p binding site in the 3’UTR sequences of Ndfip1. B MiR-155-5p negatively regulated the luciferase activity of Ndfip1-3’UTR-wt, rather than Ndfip1-3’UTR-wt in 293T cells. C and D Relative Ndfip1 expression was compared by Western blot analysis. β-actin is an internal reference. E–G The expression of PTEN in the cytoplasm and nucleus of was astrocytes compared by Western blot analysis. β-actin and Lamin B served as cytoplasmic and nuclear internal references, respectively. Values are means ± SD. H and I Immunofluorescence staining for PTEN and Ndfip1 in astrocytes. Scale bars = 50 μm. *P < 0.05 vs. control, **P < 0.01 vs. control, ^#P < 0.05 vs. miR-155-5p mimic (low), ^##P < 0.01 vs. miR-155-5p mimic (low)