Fig. 1. Properties of cation transport of ATP13A2.

a Thapsigargin (TG)-sensitive ATPase activity in the ATP13A2- and mock-transfected HEK293 cells. (n = 4 independent replicates).　Insert shows Western blot images of ATP13A2 (~150 kDa) and Na⁺,K⁺-ATPase (NaK; ~100 kDa). Statistical significance was determined by two-tailed unpaired Student’s t test. b ΔTG-sensitive ATPase activities (the difference between 50 nM TG- and 5 μM TG-sensitive activities) in the ATP13A2 WT-, D513N- and mock-transfected cells. (n = 4–6 independent replicates). Insert shows Western blot images of ATP13A2 (~150 kDa) and Na⁺,K⁺-ATPase (NaK; ~100 kDa). Statistical significance was determined by two-tailed unpaired Student’s t test. c ΔTG-sensitive ATPase activities in the ATP13A2-expressing HEK293 cells at various concentrations of Na⁺ and K⁺. (n = 3–5 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. d K⁺-dependent activation of ΔTG-sensitive activity in the ATP13A2- and mock-transfected cells. (n = 4 independent replicates). Statistical significance was determined by one-way ANOVA. e ATP-dependent ⁸⁶Rb⁺ efflux from lysosomes of the ATP13A2- and mock-transfected HEK293 cells. Effect of TG (5 μM) on the ⁸⁶Rb⁺ efflux was examined. (n = 4–5 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. f Time-dependence of the ⁸⁶Rb⁺ efflux in the ATP13A2-transfected HEK293 cells. (n = 4 independent replicates). g pH-dependence of ΔTG-sensitive activity in the ATP13A2- and mock-transfected cells. (n = 4 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. h, i Effects of Mn²⁺ (50 μM), Fe²⁺ (50 μM), Zn²⁺ (50 μM) (h), and Ca²⁺ (0 and 20 μM) (i) on ΔTG-sensitive ATPase activity in the ATP13A2-transfected cells. (n = 4 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. NS, P > 0.05. j Effect of spermidine (SPD; 1 mM) on ΔTG-sensitive ATPase activity in the ATP13A2- and mock-transfected HEK293 cells. (n = 5 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. k Effect of spermidine (SPD; 1 mM) on the ATP-dependent ⁸⁶Rb⁺ efflux in lysosomes of the ATP13A2-transfected HEK293 cells. (n = 4-6 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. All data are presented as mean ± SEM. Source data are provided as a source data file.