Fig. 3. Effects of inhibitors of gastric H⁺,K⁺-ATPase (P-CABs) and vacuolar H⁺-ATPase on exogenous ATP13A2 activity in HEK293 cells.

a–c Concentration-dependent effects of SCH28080 (a), vonoprazan (b), and bafilomycin A1 (c) on the ΔTG-sensitive ATPase activity in the ATP13A2- and mock-transfected HEK293 cells. (n = 3–6 independent replicates). Statistical significance was determined by one-way ANOVA. d Effects of SCH28080 (SCH; 10 μM), vonoprazan (Vono; 10 μM), bafilomycin (BFA; 5 nM) on the ATP-dependent ⁸⁶Rb⁺ efflux from lysosomes of the ATP13A2-transfected HEK293 cells. (n = 4–5 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. e Effects of SCH28080 (SCH; 50 μM), vonoprazan (Vono; 50 μM), and bafilomycin A1 (BFA; 5 nM) on the lysosomal pH. In the ATP13A2- and mock-transfected HEK293 cells, the lysosomal pH was measured at 0 and 20 min after treatment of each drug. Then, the pH difference (ΔpH_lyso) between 0 and 20 min was calculated. (n = 3–6 independent replicates). Statistical significance was determined by two-tailed unpaired Student’s t test. All data are presented as mean ± SEM. Source data are provided as a source data file.