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. 2023 Apr 20;13:6445. doi: 10.1038/s41598-023-32897-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rate of mitochondrial oxidation (a) and retinal oxygenation (b) measured at t = 15 min for all the subjects with age (N = 30). (a) There is a mild-moderate age-related decline in CCO oxidation rate (rate of change in concentration of oxidized cytochrome-c-oxidase (Δ[oxCCO]) in dark (Pearson correlation coefficient R = −4 and coefficient p value, p = 0.03). (b) Rate of hemoglobin oxygenation (rate of change in hemoglobin difference (Δ[HbDiff])) has a mild positive association with age which is not statistically significant (Pearson correlation coefficient R = 0.3 and coefficient p value, p = 0.06).