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. 2023 Feb 6;26(2):217–232. doi: 10.1007/s10456-023-09869-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Gltscr1-deficient embryo results in cardiac defects. A Genotyping analysis from Gltscr1^+/− and Gltscr1^+/− interbreeding. B Representative images of gross appearance in Gltscr1^+/+, Gltscr1^+/−, and Gltscr1^−/− embryos at E10 to E15. The arrow indicates the peripheral edema in Gltscr1^−/−. Scale bar: 5 mm. C Representative images of gross appearance in Gltscr1^+/+, Gltscr1^+/−, and Gltscr1^−/− embryos at E13.5. The dashed area indicates the heart area. Scale bar: 10 mm. D Representative H&E staining images of embryonic heart tissues collected at E11.5, E13.5, and E15 from Gltscr1^+/+ and Gltscr1^−/− embryos. Scale bar: 500 µm. E Quantification of compacted cardiomyocyte (CM) (D) was performed using ImageJ software. Data were shown as the mean ± S.D.; n = 18 sections from 3 independent experiments. *P < 0.05 compared to control (two-tailed Student’s t test were used)