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. 2023 Apr 21;14(4):281. doi: 10.1038/s41419-023-05787-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Dose-response toxicity to iFSP1 in Pfa1 Gpx4^−/− overexpressing hFSP1, mFSP1 and single point mutants (mFSP1^K319Q, mFSP1^L360F and hFSP1^F360L). B Binding modes for iFSP1 obtained from docking the ligand to hFSP1 WT or to different mutants. The backbone of hFSP1 WT is represented in green. Cyan: iFSP1 bound to hFSP1 WT, dark pink: iFSP1 bound to mutants F360L or F360I, light pink: iFSP1 bound to mutants Y296A or F360I/Y296A. Only one FSP1 structure (hFSP1 WT) is represented. Interaction diagrams between iFSP1 and hFSP1 WT (C), mutant F360L (D) and mutant Y296A (E). Purple arrows indicate hydrogen bonds. F Dose-response toxicity to iFSP1 in Pfa1 overexpressing hFSP1, mFSP1 and the mutants hFSP1^Y296A, hFSP1^F360A and hFSP1^Y296A/F360A. G Immunoblot analysis of flag-tag, GPX4 and FSP1 in Pfa1 Gpx4^−/− overexpressing hFSP1, mFSP1 and mutants. Cell viability data was monitored using Alamar blue. Data are presented as mean ± s.d. of n = 3 wells of a 96-well plate from one representative of three independent experiments; *p < 0.05; two-way analysis of variance (ANOVA).