Fig. 1.

E-MET improves L-MET effects decreasing susceptibility of Epm2a^−/− and Epm2b^−/− mice to PTZ-induced seizures. We analyzed the percentage of animals with myoclonic jerks at a PTZ dose of 30 mg/kg (a) and the latency to myoclonic jerks at a PTZ dose of 50 mg/kg (b) in 12-month-old wild type (WT), Epm2a^−/− and Epm2b^−/− mice. We also evaluated the percentage of mice with PTZ-induced GTC seizures (c), their latency (d), length (e), and lethality (f), induced by a PTZ dose of 50 mg/kg in 12-month-old mice. Only one mouse treated with L-MET had a GTC seizure. When data are shown as a percentage, a Fisher’s exact test was performed between experimental groups. When data are shown as a mean ± SEM, a two-way ANOVA test was performed with Turkey’s multiple comparisons between the experimental groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001