Fig. 4.

E-MET improves the effect of L-MET on the reduction of neurodegeneration, astrogliosis, and formation of LBs in 12-month-old Epm2a^−/− and Epm2b^−/− mice. We studied the number of NeuN and GFAP positive cells and LBs positive to PAS-D staining, in the CA1 area of the hippocampus in WT, Epm2a^−/− (a, b) and Epm2b^−/− mice (c, d). Data are shown as a normalized mean ± SEM. Means were normalized using WT control values in NeuN and GFAP immunostaining assays, while the numbers of LBs detected in untreated Epm2a^−/− or Epm2b^−/− mice were used to normalize values in PAS-D staining. A Kruskal–Wallis non-parametric test followed by a Dunn’s multiple comparison test was performed. *p < 0.05; scale bar = 100 µm. NeuN, neuronal nuclear antigen; GFAP, glial fibrillary acidic protein; PAS-D, periodic acid–Schiff (PAS) stain used in combination with diastase