Fig. 1.

In vivo multi-photon microscopy to visualize inflammatory responses. a Principle of multi-photon microscopy. b In vivo visualization of immune cell dynamics in the spinal cord of a triple fluorescent mouse during EAE progression (d, day after EAE induction; modified from [57]). c Quantification of the same inflammatory plaque shown in b. d 3D-multicolor 3P- and third harmonic generation (THG) images of the cortex of a multifluorescent mouse illustrating the typical 1.2 mm imaging depth achieved at 1340 nm excitation as well as the THG signal collected from corpus callosum myelin (modified from [84])