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(A) SELDI-TOF-MS spectrums of fresh (upper) and frozen-thawed (bottom) sperm samples. (B) SDS-PAGE analysis of fresh and frozen-thawed sperm samples to show total expression patterns of proteins eluted in NaCl solution. SELDI-TOF MS, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry; SDS-PAGE, sulphate polyacrylamide gel electrophoresis.
