Table 1. A list of proteins with increased expression levels in fresh and frozen-thawed boar spermatozoa.

No.	Accession	Entry name	Protein name	Gene name	Fold change (log2)
Fresh spermatozoa
1	F1SS24	F1SS24_PIG	Uncharacterized protein	FN1	31.3991
2	O02705	HS90A_PIG	Heat shock protein HSP90-alpha	HSP90AA1	13.4940
3	P26322	AQN1_PIG	Carbohydrate-binding protein AQN-1	AQN-1	3.4567
4	P26776	AWN_PIG	Carbohydrate-binding protein AWN	AWN	2.9554
5	I3LHM6	I3LHM6_PIG	Alpha-mannosidase	MAN2B2	2.8808
6	F1RI24	F1RI24_PIG	CUB domain-containing protein	PSP-I	2.4616
7	P24020	AQN3_PIG	Carbohydrate-binding protein AQN-3	AQN-3	2.3824
8	F1SLF0	F1SLF0_PIG	Uncharacterized protein	SPR	1.6061
9	F1RPK0	A0A480ET88_PIG	Histone H2A	BN2614_LOCUS10	1.5845
Frozen-thawed spermatozoa
1	F1SJ34	F1SJ34_PIG	Cytochrome c oxidase subunit 5A, mitochondrial	COX5A	−1.5385
2	P79385	MFGM_PIG	Lactadherin	MFGE8	−1.5742
3	F1SF28	F1SF28_PIG	Zona pellucida-binding protein 1	ZPBP	−1.5919
4	I3LVQ4	A0A481AMS1_PIG	Uncharacterized protein	NCOA1	−1.5966
5	F1S605	F1S605_PIG	Glutathione S-transferase	GSTM3	−1.6121
6	A9Y007	A9Y007_PIG	Aquaporin 7	AQP7	−1.6396
7	D5K8A9	SACA1_PIG	Sperm acrosome membrane associated protein 1	SPACA1	−1.6822
8	I3LMP2	I3LMP2_PIG	Uncharacterized protein	ABHD10	−1.7676
9	F1RJI7	F1RJI7_PIG	Uncharacterized protein	Cox6a1	−1.7862
10	F1SSA1	F1SSA1_PIG	Chromosome 1 open reading frame 56	C1orf56	−1.7940
11	F1RRW4	F1RRW4_PIG	Angiotensin-converting enzyme	ACE	−1.8385
12	F2Z536	F2Z536_PIG	Dynein light chain	DYNLL2	−1.9144
13	I3LK59	I3LK59_PIG	Alpha-enolase isoform 1	ENO1	−1.9462
14	F1SIS9	F1SIS9_PIG	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial	NDUFA10	−1.9713
15	Q8MI02	Q8MI02_PIG	Hyaluronidase	SPAM-1	−2.0370
16	F1SQN1	F1SQN1_PIG	T-complex protein 1 subunit delta	CCT4	−2.0600
17	F1RT87	F1RT87_PIG	Ras-related protein Rab-2A isoform a	RAB2A	−2.1284
18	F1RQI1	F1RQI1_PIG	Cytochrome c oxidase subunit 7A2, mitochondrial	COX7A2	−2.1484
19	P00571	KAD1_PIG	Adenylate kinase isoenzyme 1	AK1	−2.1770
20	D9U8D1	D9U8D1_PIG	Dynein light chain	LC8	−2.1872
21	F1RI18	F1RI18_PIG	Uncharacterized protein	UQCRQ	−2.2569
22	F1RRQ6	F1RRQ6_PIG	LRRC37AB_C domain containing protein	LOC102158372	−2.2789
23	F1SLA0	F1SLA0_PIG	ATP synthase subunit beta	ATP5F1B	−2.3036
24	Q29549	CLUS_PIG	Clusterin	CLU	−2.3829
25	F1SM79	F1SM79_PIG	Uncharacterized protein	PKDREJ	−2.4067
26	F1RPS8	F1RPS8_PIG	ATP synthase subunit alpha	ATP5F1A	−2.4819
27	F1SDN2	F1SDN2_PIG	Trifunctional enzyme subunit beta, mitochondrial isoform X1	HADHB	−2.5090
28	P00348	HCDH_PIG	Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial	HADH	−2.5180
29	I3LB44	I3LB44_PIG	Malate dehydrogenase	ENSSSCG00000022810	−2.5453
30	Q28983	ZAN_PIG	Zonadhesin	ZAN	−2.5518
31	C1JEX7	C1JEX7_PIG	Sperm associated antigen 6	SPAG6	−2.5748
32	F2Z5G3	F2Z5G3_PIG	Calmodulin-2 isoform 1	CALM2	−2.5810
33	F1SD45	F1SD45_PIG	cAMP-dependent protein kinase catalytic subunit alpha	PRKACA	−2.6196
34	F1SLY2	A0A4X1VWT5_PIG	Uncharacterized protein	NDUFA5	−2.6405