REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Keratinocyte serum-free media (K-SFM)	Thermo Fisher Scientific	17005042
Renal Epithelial Cell Growth Medium (REGM)	Lonza	CC-3190
Penicillin-Streptomycin	Thermo Fisher Scientific	15140122
Collagenase IV	Sigma-Aldrich	C5138
Collagen Coating Solution	Sigma-Aldrich	125-50
XF base medium without phenol red	Agilent	103335-100
Oligomycin	Sigma-Aldrich	75351
Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP)	Sigma-Aldrich	C2920
Rotenone	Sigma-Aldrich	R8875
Antimycin A	Sigma-Aldrich	A8674
Seahorse XF Plasma Membrane Permeabilizer	Agilent	02504-100
D-Glucose-6-Phosphate sodium salt (G6P)	Sigma-Aldrich	G7879
D-Fructose 1,6-bisphosphate trisodium salt hydrate (F1,6BP)	Sigma-Aldrich	F6803
D-Glyceraldehyde-3-phosphate solution (G3P)	Sigma-Aldrich	39705
D-(-)-3-Phosphoglyceric Acid (sodium salt) (3PG)	Cayman Chemical	20123
Phospho(enol)pyruvic acid monosodium salt hydrate (PEP)	Sigma-Aldrich	P0564
L-(+)-Lactic acid	Sigma-Aldrich	L1750
Glucose	Thermo Fisher Scientific	A24940-01
2-deoxy-D-glucose (2-DG)	Sigma-Aldrich	D8375
Critical commercial assays
Seahorse XF FluxPak (cartridges, microplates, calibrant)	Agilent	02416-100
Seahorse XF Real-Time ATP Rate Assay Kit	Agilent	103592-100
Pierce™ BCA Protein Assay Kit	Thermo Fisher Scientific	23227
Experimental models: Cell lines
Human kidney-2 (HK-2), Male	ATCC	CRL-2190, RRID:CVCL_0302
Human Renal Proximal Tubule Epithelial Cells (RPTEC), Female	Lonza	CC-2553-0000560580
Rat glomerular visceral epithelial cells (podocytes)	Laboratory of Dr. Yves Gorin	N/A (details published in Mol Cell Biol 2013 Vol. 33 Issue 17 Pages 3439-60- https://doi.org/10.1128/MCB.00217-13)
Rat mesangial cells	Laboratory of Dr. Yves Gorin	N/A (details published in PLoS One 2018 Vol. 13 Issue 11 Pages e0207285- https://doi.org/10.1371/journal.pone.0207285)
C2C12, Female	ATCC	CRL-1772, RRID:CVCL_0188
Experimental models: Organisms/strains
C57BL/6J mice, males	The Jackson Laboratory	RRID:IMSR_JAX:000664
BKS.Cg-Dock7m +/+ Leprdb/J mice (db/db), males	The Jackson Laboratory	RRID:IMSR_JAX:000642
Software and algorithms
Agilent Seahorse XF WAVE	Agilent	https://www.agilent.com
Seahorse XF Real-Time ATP Rate Assay Report Generator	Agilent	https://www.agilent.com
Mass Hunter Q-TOF Quantitative Analysis version B.07.01 Software	Agilent	https://www.agilent.com
Prism 9 GraphPad	GraphPad	https://www.graphpad.com
Other
Seahorse XFe96 Analyzer	Agilent	N/A
Agilent 6456 quadrupole time-of-flight (Q-TOF) mass spectrometer coupled to Agilent 1290 High-performance liquid chromatography	Agilent	N/A