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. 2023 Mar 23;26(4):106478. doi: 10.1016/j.isci.2023.106478

Figure 3.

Figure 3

Small interfering (si)RNA knockdown of c-MAF in IEC-18 cells

(A) c-MAF siRNA or negative control siRNA was transfected into rat intestinal IEC-18 cells at 30 nM c-MAF mRNA expression was measured by qRT-PCR. (n = 3).

(B) Knockdown of c-MAF expression led to increased cell proliferation. (n = 3).

(C) Colony-forming ability was significantly increased in c-MAF knockdown cells compared with negative control cultures. (n = 3) Left panel: representative pictures of Giemsa staining of colonies in the 6-well plate. Data are expressed as the mean ± standard deviation. Statistical differences were analyzed by the Student’s t test. ∗p < 0.05. Si-MAF, c-MAF-siRNA; NC, negative control siRNA.