Table 2.
Lists of activated molecules in normal mucosa of c-MAF KO mice compared with wild-type mice after treatment with AOM/DSS
| Upstream Regulator | Molecule Type | Predicted Activation State | Activation Z score | p value |
|---|---|---|---|---|
| ESR1 | Ligand-dependent nuclear receptor | Activated | 4.146 | 0.068 |
| ERBB2 | Kinase | Activated | 3.762 | 0.000 |
| EGF | Growth factor | Activated | 3.253 | 0.029 |
| E2F1 | Transcription regulator | Activated | 3.179 | 0.002 |
| IL4 | Cytokine | Activated | 3.160 | 0.016 |
| Insulin | Group | Activated | 3.098 | 0.239 |
| MITF | Transcription regulator | Activated | 3.073 | 0.003 |
| CD3 | Complex | Activated | 3.054 | 0.012 |
| CEBPB | Transcription regulator | Activated | 3.035 | <0.001 |
| RABL6 | Other | Activated | 2.828 | < 0.001 |
| ESR2 | Ligand-dependent nuclear receptor | Activated | 2.724 | 0.358 |
| NTRK2 | Kinase | Activated | 2.599 | < 0.001 |
| IL13 | Cytokine | Activated | 2.576 | 0.024 |
| CKAP2L | Other | Activated | 2.449 | < 0.001 |
| MYB | Transcription regulator | Activated | 2.449 | 0.010 |
| TAL1 | Transcription regulator | Activated | 2.449 | 0.081 |
| FOXM1 | Transcription regulator | Activated | 2.441 | 0.024 |
| INSR | Kinase | Activated | 2.433 | 0.019 |
| CD38 | Enzyme | Activated | 2.425 | 0.025 |
| CCR2 | G-protein coupled receptor | Activated | 2.412 | 0.004 |
| AIRE | Transcription regulator | Activated | 2.401 | 0.001 |
| RORA | Ligand-dependent nuclear receptor | Activated | 2.236 | 0.027 |
| CYP1B1 | Enzyme | Activated | 2.219 | 0.018 |
| IL33 | Cytokine | Activated | 2.170 | 0.005 |
| CCND1 | Transcription regulator | Activated | 2.101 | < 0.001 |
| E2f | Group | Activated | 2.034 | < 0.001 |
| STAT5a/b | Group | Activated | 2.000 | 0.090 |
| Eldr | Other | Activated | 2.000 | 0.007 |
| TFDP1 | Transcription regulator | Activated | 2.000 | < 0.001 |
| NKX2-3 | Transcription regulator | Activated | 2.000 | 0.339 |
| TPH1 | Enzyme | Activated | 2.000 | 0.025 |
| BRCA1 | Transcription regulator | Activated | 2.000 | 0.100 |
| CDK2 | Kinase | Activated | 2.000 | < 0.001 |
Cancer-promoting growth factors, transcription factors, kinases, and cytokines are shown in bold.
Ingenuity Pathway Analysis of normal mucosa showed that many cancer-promoting growth factors, transcription factors, kinases, and cytokines were activated as upstream regulators in c-MAF KO mice.