Figure 1.

Induction of Gα_s^R201C in osteoprogenitors induces osteoclast formation in marrow cultures

(A) A cartoon schematic the genetic tools utilized and our approach for implementing them to model oteoprogenitor-osteoclast signaling/coordination in the formation of osteoclasts.

(B) A representative Western blot evaluating Gα_s expression with (Dox.) or without (Unind.) 4 days induction.

(C) ELISA evaluation of soluble RANKL in the culture media of marrow explants with (Dox.) or without (Unind.) 4 days induction. (n = 7).

(D) ELISA evaluation of soluble IL-6 in the culture media of marrow explants with (Dox.) or without (Unind.) 4 days induction. (n = 4).

(E) ELISA evaluation of soluble M-CSF in the culture media of marrow explants with (Dox.) or without (Unind.) 4 days induction. (n = 5).

(F) Representative color brightfield images of marrow explants from Gα_s^R201C following 4 days with (Dox.), without (Unind.) or with induction in the presence of OPG (Dox.+OPG). Arrow denotes x-gal staining of the nucleus of an induced osteoprogenitor, Arrowhead denotes a multinucleated, TRAP⁺ osteoclast. Error bars = ±SEM. Significance was assessed via paired t-test, where ∗p=<0.05, ∗∗p=<0.01, ∗∗∗p=<0.001.