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. 2023 Mar 23;26(4):106470. doi: 10.1016/j.isci.2023.106470

Figure 4.

Figure 4

Osteoclast formation in ex vivo marrow cultures is accompanied by EV release

(A) An illustrated schematic depicting an approach for enriching an EV fraction from uninduced, Doxy. induced and Doxy. induced +100 ng/mL OPG ex vivo marrow cultures (left). A representative Western blot evaluating EV enriched fractions for the osteoclast membrane receptor RANK and the exosome marker TSG101 (right).

(B) Quantification of RANK signal in representative Western blots of EV enriched fractions, as in (A). (n = 3).

(C) Quantification of TSG101 signal in representative Western blots of EV enriched fractions, as in (A). (n = 5) Error bars = ±SEM. Significance was assessed via paired t-test, where ∗p=<0.05, ∗∗p=<0.01, ∗∗∗p=<0.001.