Figure 5.
Ex vivo FD marrow explants as a model of osteoclast formation and osteoclast-osteoprogenitor coordination
An illustration of the biological process modeled in our ex vivo marrow cultures. Doxycycline induction elicits osteoprogenitor expression of GαsR201C and release of RANKL. RANKL binding initiates osteoclastogenesis and the formation of multinucleated, bone resorbing osteoclasts. Osteoclasts release EVs that correlate with preosteoblast proliferation. Peach cells are of the monocyte>osteoclast lineage. Blue cells are of the skeletal stem cell>osteoblast lineage.