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. 2022 Nov 1;7(7):1312–1325. doi: 10.1182/bloodadvances.2022007719

Figure 1.

Figure 1.

CD11c is expressed in neutrophils intracellularly. (A) Flow cytometry gating strategy identifying preneutrophils and immature and mature neutrophils in mouse BM. From the side scatter–A and forward scatter–A plot, live cells were selected (supplemental Figure 1A). Here, Lin includes CD3e, B220, and NK1.1. (B) Surface and intracellular CD11c expression detected by flow cytometry. Shown are representative data of at least 5 independent experiments with the same pattern. (C) Fluorescence microscopic examination (×60) of BM neutrophils. MPO, red; 4′,6-diamidino-2-phenylindole, blue; CD11c, green. Images show representative images of 3 independent experiments. c, conventional; p, plasmacytoid.