Figure 2.
CDC25A silencing inhibited the proliferation of HK1 cells. (A) The protein expression of CDC25A was determined by western blotting. (B) The relative mRNA expression of CDC25A was quantified by reverse transcription-quantitative PCR. (C) HK1 cell viability was measured by CCK8 assay. (D) The expressions of Ki67 and PCNA were determined by western blotting. The data were expressed as mean ± standard error of the mean of three independent experiments. **P<0.01, ***P<0.001 vs. siRNA-NC. CDC25A, cell division cycle gene 25A; PCNA, proliferating cell nuclear antigen.