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. 2023 Apr 12;27(5):109. doi: 10.3892/mmr.2023.12996

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — CDC25A was transcriptionally regulated by E2F1 in HK1 cells. (A) The interaction between CDC25A and E2F1. (B) The search results from Cyclebase database. (C) The binding sites of CDC25A promoter and E2F1. The mutational fragments are in red. (D) The protein expression of E2F1 was determined by western blotting. (E) The relative mRNA expression of E2F1 was quantified by RT-qPCR. (F) The protein expression of CDC25A was determined by western blotting. (G) The relative mRNA expression of CDC25A was quantified by RT-qPCR. (H) The protein expression of E2F1 was determined by western blotting. (I) The relative mRNA expression of E2F1 was quantified by RT-qPCR. (J) The protein expression of CDC25A was determined by western blotting. (K) The relative mRNA expression of CDC25A was quantified by RT-qPCR. (L) The interaction between CDC25A and E2F1 was confirmed by luciferase reporter assay and ChIP assays. In luciferase reporter assay, FL group used the normal FL of CDC25A promoter while Site 1 and Site 2 groups used the FL of CDC25A promoter including the two mutational sequences of CDC25A promoter. (M) The interaction between CDC25A and E2F1 was confirmed by ChIP assay. The data were expressed as mean ± standard error of the mean of three independent experiments. ***P<0.001 vs. Control. CDC25A, cell division cycle gene 25A; E2F1, E2F transcription factor 1; RT-qPCR, reverse transcription-quantitative PCR; ChIP, chromatin immunoprecipitation; FL, full length.