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TIL reactivity toward spliced peptides. 4-1BB expression was detected in TILs following 24 h stimulation with 1 μg/ml DAPK1 (LLLDKLLSV), HLA-E (WSDSSGGKGGSY), NRAS Q61K (ILDTAGKEEY) or dimethyl sulfoxide (control), using flow cytometry analysis. T cell reactivity was validated by ≥2 independent experiments.
