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. 2023 Feb 23;22(4):100519. doi: 10.1016/j.mcpro.2023.100519

Fig. 4.

Fig. 4

HLA-I dextramer staining of DAPK1-specific CD8 T cells. T cells derived from healthy donor PBMCs were stimulated with 721.221 B-LCLs expressing the HLA-A∗02:01 allele pulsed with 10 μg/ml DAPK1 or 10 μg/ml viral peptide (control). Following expansion, T cells were stained with DAPK1 dextramers to evaluate the percentage of DAPK1-specific subpopulations. CD8 T cells were gated as single, live, CD3 positive and CD8 positive cells. The images are representative of two replicates. PBMC, peripheral blood mononuclear cell.