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. 2023 Mar 23;26(4):106492. doi: 10.1016/j.isci.2023.106492

Figure 3.

Figure 3

Characterization of FR and YM derivatives in radioligand-binding assays versus [³H]PSB-15900

Competition-binding experiments of (A) FR and its derivatives, (B) YM and its derivatives YM-1, YM-3, YM-7, and YM-8, (C) YM and its derivatives YM-9 to YM-12, and (D) YM and its derivatives YM-13, YM-14, YM-15, and YM-18 versus [³H]PSB-15900.

(E) pKi values of FR, YM, and all investigated derivatives as determined in competition-binding assays. For affinity values see Table 1. Significance levels were obtained from a one-way ANOVA with multiple comparisons (FR derivatives were compared to FR, YM derivatives were compared to YM) using Dunnett’s post-hoc test; p > 0.05 not significant (n.s.), p < 0.05 ∗; p < 0.01 ∗∗; p < 0.001 ∗∗∗. Data is represented as mean ± SD of three separate experiments, performed in duplicates.

(F) Correlation of affinity data from competition-binding assays with functional data from previously performed IP1 assays; slope = 0.75, R2 = 0.89,20–22 an ideal line (slope = 1) is plotted in light gray for comparison.