Fig. 1. Major pulmonary cell types emerge at the initiation of human embryonic lung development.

a Schematic overview of scRNA-seq experimental design focusing on embryonic and pseudoglandular stages of human lung development. Sample images are presented in Supplementary information. Fig. S1b. b t-SNE projection visualizing 169,686 human embryonic lung cells, clustered into six major cell types. c Dot plot showing the top 2 cell type marker expression. The size and color of each dot represent the expression percentage and average expression of the indicated gene in each cell type, respectively. d Pie plot showing the proportions of six major cell types. e t-SNE layout mapping the distribution of single-cell profiles of each time point denoted as the embryonic week (wk).