Fig. 1.

Loss of murine endothelial CSE reduces TFPI secretion and S-sulfhydration.

(A) TFPI, CSE and GAPDH levels in pulmonary endothelial cells from wild-type (WT) and endothelial cell-specific CSE knockout (iEC) littermates at passage 5; n = 6 independent cell batches/group. (B) Polysulfide levels (H₂S_n) in cells as in panel A; n = 6 independent cell batches/group. (C) S-sulfhydration of TFPI (TFPI-SSH) detected as the relative fluorescence intensity of Daz2:Cy5/NBF in endothelial cells and plasma from WT and iEC mice; n = 6 mice/group. (D) Endothelial cell (left panel) and plasma (right panel) levels of TFPI in WT and iEC mice treated with vehicle or SG1002 (40 mg/kg/day in diet) for 2 weeks; n = 6 mice/group. ***P < 0.001, Student's t-test (A, B), one-way ANOVA and Bonferroni multiple comparisons test (C, D).