Figure 1.

Development of DNA aptamers to postoperative gliomas

(A) The scheme of an aptamer selection procedure. (B) Flow cytometry analysis of binding properties of aptamer pools. (C) Flow cytometry analysis of binding properties of individual aptamers: comparison of different aptamers binding to glial and non-glial brain tumors (1); fluorescence intensity of glial tumor cells attached with the aptamers Gli-233 (4) and Gli-7819 (5) comparing with non-stained control (2) and nonspecific oligonucleotide (3). (D) Brain tumor tissue sections: stained with H&E dyes (1); stained with Cy5-labeled aptamers: Gli-233 (2); Gli-7819 (3), Gli-55 (4). Magnification, 40×. n = 3, Standard deviation, Scale bar, 50 µm