Figure 2.

Binding verification of Gli-233 to tubulin and Gli-55 to GFAP

(A) Co-staining with Alexa Fluor 405-labeled anti-tubulin antibodies and Cy5-labeled Gli-233 of glioma tissue sections. (B) Co-staining with Alexa Fluor 405-labeled anti-tubulin antibodies and Cy5-labeled Gli-233 of primary GBM cell cultures. (C) Co-staining with Alexa Fluor 405-labeled anti-GFAP antibodies and Cy5-labeled Gli-55 of glial tumor tissue sections. Overlays (A3, B3, C3) and histograms of overlapping fluorescence intensities in the depicted regions (A4, B4, C4). The coinciding peaks are shown with black arrows; peaks that do not match are shown with green arrows. Magnification, 40×. Scale bars, 20 µm, 50 µm, 100 µm.