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MHC-I evasion by SARS-CoV-2 variants. (A) Calu-3 cells were infected with SARS-CoV-2 variants at MOI 0.3 for 40 h. The cell surface expression of HLA-ABC was analyzed by flow cytometry. (B and C) Calu-3 cells were infected with SARS-CoV-2 variants at MOI 0.01 for 48 h. The mRNA levels of HLA-A, -B, -C (B), NLRC5, and IRF1 (C) were measured by qRT-PCR. Data are mean ± SD. Data are representative of two to three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.
