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. 2023 Apr 11;120(16):e2300099120. doi: 10.1073/pnas.2300099120

Fig. 3.

Fig. 3.

Depletion of CD4+ T Cells Overcomes the Effect of Ctsb Deficiency on HEL-Binding B Cells. (A) Schematic of CTV-labeled MD4 B cell adoptive transfer into control or Ctsb-deficient mice, followed by GK1.5 CD4+ T cell depletion and HEL treatment. (B) Frequencies of transferred MD4 B cells in spleens of control or Ctsb-deficient recipients 3 d after saline (n = 6 control), HEL (n = 7 control, n = 9 KO), or HEL with GK1.5 treatment (n = 6 control, n = 9 KO). (C–E) MFI (Top) and representative histogram plot (Bottom) of CD23 (C), ICAM1 (D), and IgMa (E) on transferred MD4 B cells 3 d after saline (n = 5 control), HEL (n = 6 control, n = 5 KO), or HEL with GK1.5 treatment (n = 6 control, n = 5 KO). (F) Percentage of divided transferred MD4 B cells (Top) or representative histogram plot of CTV (bottom) 3 d after saline (n = 3 control, n = 2 KO), HEL (n = 6 control, n = 6 KO), or HEL with GK1.5 treatment (n = 6 control, n = 5 KO). Each data point indicates an individual mouse and lines indicate means. Error bars represent SDs. C–F are representative of three experiments. Statistical significance for B–F was determined by unpaired t test. NS, not significant; *P < 0.05; **P < 0.01, ***P < 0.001.