Figure 1.

Western blot analysis of sOva encoding AAV preparations

Vector genome-containing particles (1 × 10¹⁰) of the indicated AAV preparations, encoding for sOva, were separated on an 8% SDS-PAGE. The capsid protein-specific antibody B1 was used for visualizing the three different capsid proteins VP1, VP2, and VP3 in their wild-type as well as modified form (VP1, 87 kDa; VP2, 72 kDa; VP3, 62 kDa; Ova8+4-eGFP-VP2, 98 kDa; cOva-VP2, 96 kDa). AAV-Vac_Ova4⁵⁸⁷ or AAV-Vac_Ova8⁵⁸⁷ encoding for sOva were produced as hybrid capsids of wild-type and epitope-containing capsid proteins (MHC class I Ova epitope in the case of AAV-Vac_Ova8⁵⁸⁷ and MHC class II Ova epitope in the case of AAV-Vac_Ova4⁵⁸⁷). Therefore, double bands are visible in lanes 3 and 4.