Figure 2.

Presence of both capsid-displayed MHC class I- and MHC class II-restricted epitopes is more effective in supporting the induction of adaptive immune responses than the presence of either epitope

Experimental design as depicted (A). C57BL/6j mice (7 to 8 weeks old; n = 7 per group) received via intramuscular injection AAV2::sOva, AAV-Vac_Ova4⁵⁸⁷, or AAV-Vac_Ova8⁵⁸⁷ at a dose of 3 × 10⁹ vector genome-containing particles (vg) per animal, or a 1:1 mixture of AAV-Vac_Ova4⁵⁸⁷ and AAV-Vac_Ova8⁵⁸⁷ (termed AAV-Vac_Ova4+8⁵⁸⁷) at a total dose of 6 × 10⁹ vg per animal. Particles encoding for sOva (see Table 1). Blood and serum samples were collected on days (D) 14, 21, 43, and 63. Blood samples were evaluated by flow cytometry after staining with H-2K^b/Ova_257-264 dextramer for the presence of Ova-specific CD8⁺ T lymphocytes (B): D14 + 21 + 63, AAV2::sOva, AAV-Vac_Ova4⁵⁸⁷, and AAV-Vac_Ova8⁵⁸⁷ n = 6; D43, AAV2::sOva and AAV-Vac_Ova4⁵⁸⁷ n = 5 and Vac_Ova8⁵⁸⁷ n = 6, and serum samples by ELISA for the presence of Ova-specific IgG antibodies (C). Statistical analysis: Kruskal-Wallis test with Dunns post test; ∗p ≤ 0.05, ∗∗p ≤ 0.01. Data are represented as mean with SEM.