Fig. 2.

UL56-deficiency enhances HSV-1-induced antiviral response. (A) UL56-deficiency enhances HSV-1-induced transcription of antiviral genes. The indicated cells were infected with HSV-1 (MOI=1) or HSV-1ΔUL56 (MOI=1) for the indicated times before qPCR analysis. (B-C) UL56-deficiency enhances HSV-1-induced phosphorylation of MITA, TBK1, and IRF3. THP-1 and MLF cells were infected with HSV-1 (MOI=1) or HSV-1ΔUL56 (MOI=1) for the indicated times before immunoblot analysis was performed with the indicated antibodies (B). The lower panels were results of RT-PCR analysis for viral and cellular mRNA (C).

The data shown are means ​± ​SD (A) from one representative experiment performed in triplicates (3 technical repeats). Similar data were obtained from at least two independent experiments. ∗, p ​< ​0.05; ∗∗, p ​< ​0.01 (Student's unpaired t-test).