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. 2022 Feb 12;1(2):100014. doi: 10.1016/j.cellin.2022.100014

Fig. 4.

Fig. 4

UL56 interacts with cGAS. (A) Association of UL56 with cGAS. HEK293T cells were transfected with the indicated plasmids for 24 h. Co-immunoprecipitation and immunoblot analysis were performed with the indicated antibodies. (B) Co-localization of UL56 with cGAS. HeLa cells stable expressing Flag-cGAS (green) were transfected with HA-UL56 (red) for 20 h before confocal microscopy. Nuclei were stained with DAPI (blue). The cells were observed with a Nikon confocal microscope under a 60 × oil objective. (C) Endogenous association of UL56 with cGAS following HSV-1 infection. MLFs were infected with HSV-1 (MOI=0.5) for the indicated times. Co-immunoprecipitation and immunoblot analysis were performed with the indicated antibodies. (D) UL56 directly interacts with cGAS. GST or GST-UL56 was bound to glutathione agarose beads and incubated with purified His-cGAS for 3 h. Immunoblot analysis was performed with the indicated antibodies.