Table 1.
Oligonucleotides used in this study.
| Primers | Primer sequence (5' - 3′) | Purpose |
|---|---|---|
| qEV71 PCR-F | TGAATGCGGCTAATCCCAACT | qRT-PCR |
| qEV71 PCR-R | AAGAAACACGGACACCCAAAG | qRT-PCR |
| qGAPDH PCR-F | CCCACTCCTCCACCTTTGACG | qRT-PCR |
| qGAPDH PCR-F | CACCACCCTGTTGCTGTAGCCA | qRT-PCR |
| A2747G(N104S)–F | AACCCAAgTGGTTATGCCAACTGGGACATAGA | Generate mutant virus |
| A2747G(N104S)-R | GCATAACCAcTTGGGTTAGTTGTGCCCTCAAG | Generate mutant virus |
| T3163C(S243P)–F | CCTCCAAGcCCAAGTATCCTTTAGTGGTTAGGATTT | Generate mutant virus |
| T3163C(S243P)-R | ATACTTGGgCTTGGAGGTCCCCACAGTCCGCA | Generate mutant virus |
| A2741G(N102S)–F | CACAACTAgCCCAAATGGTTATGCCAACTGGG | Generate mutant virus |
| A2741G(N102S)-R | CATTTGGGcTAGTTGTGCCCTCAAGAGGGAGA | Generate mutant virus |
| A2632G(T66A)-F | TGATTGAGgCACGCTGTGTTCTCAACTCGCAC | Generate mutant virus |
| A2632G(T66A)-R | ACAGCGTGcCTCAATCATGCTCTCGTCACTAGC | Generate mutant virus |
| G2780A(R121K)F | GCGTAaAAAGGTAGAGCTATTCACCTACATGC | Generate mutant virus |
| G2780A(R121K)R | GCTCTACCTTTtTACGCATTTGCGCGTAACCT | Generate mutant virus |
| VP1-EGFP-F | CCAAGCTTATGGGAGATAGGGTGGCAGATGTAATTG | Generate VP1 protein |
| VP2-EGFP-R | CGGGATCC CG AAGAGTGGTGATCGCTGTGCGAC | Generate VP1 protein |
| VP1-EGFP N104S–F | AACCCAAgTGGTTATGCCAACTGGGACATAGA | Generate mutant VP1 protein |
| VP1-EGFP N104S-R | GCATAACCAcTTGGGTTAGTTGTGCCCTCAAG | Generate mutant VP1 protein |
| VP1-EGFP S243P–F | CCTCCAAGcCCAAGTATCCTTTAGTGGTTAGGATTT | Generate mutant VP1 protein |
| VP1-EGFP S243P-R | ATACTTGGgCTTGGAGGTCCCCACAGTCCGCA | Generate mutant VP1 protein |
| hSCARB2-EGFP-F | CGCTCGAGATGGGCCGATGCTGCTTCTACAC | Generate EGFP-hSCARB2 protein |
| hSCARB2-EGFP-R | CCGGAATCCGGGTTCGAATGAGGGGTGCTC | Generate EGFP-hSCARB2 protein |
| Flag-VP1-F | CCAAGCTTGCCACCATGGGAGATAGGGTGGCAGATGTAATTG | Generate Flag-VP1 protein |
| Flag-VP1-R | CGGGATCCAAGAGTGGTGATCGCTGTGCGAC | Generate Flag-VP1 protein |
| HA-hSCARB2-F | CGGAATTCGGATGGGCCGATGCTGCTTCTACAC | Generate HA-hSCARB2 protein |
| HA-hSCARB2-R | CCGCTCGAGTTAGGTTCGAATGAGGGGTGCTC | Generate HA-hSCARB2 protein |