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. 2023 Mar 27;29:254–270. doi: 10.1016/j.omtm.2023.03.013

Figure 4.

Figure 4

Optimized gene delivery following the co-injection of AAV2.retro and AAV2/rh.10

(A) Schematic representation of the experimental setting. AAV2.retro-CBA-GFP and AAV2/rh.10-CBA-GFP were co-injected bilaterally into the dorsolateral striatum of mice to favor retrograde transport in the cortex and striatal transduction. (B) Dense cortical layers of GFP-positive CPNs are observed throughout the rostrocaudal axis, whereas mCherry-positive cells are widely distributed throughout the striatum. Scale bar: 1000 μm. (C) Schematic representation of the experimental setting. AAV2.retro-CBA-AcGFPnuc and AAV2/rh.10-CBA- AcGFPnuc were co-injected bilaterally into the dorsolateral striatum for the quantification of striatal transduction efficiency. (D) Coronal sections were co-registered against the Allen CCFv3 reference atlas to identify the striatum, and the 2D workflow described in Figure 3 was applied. Scale bar for low magnification: 800 μm. Scale bar for high magnification: 20 μm. (E) Quantitative analysis showing the percentage of DAPI-positive cells expressing AcGFPnuc in the left and right striatum of three animals. The mean transduction efficiency for the whole striatum is indicated. (F) Quantitative analysis showing the percentage of DAPI-positive cells expressing AcGFPnuc in five representative highly transduced areas of the dorsolateral striatum. The mean transduction efficiency in the highly transduced regions is indicated. Data are represented as mean ± SD.