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. 2023 Apr 8;27:181–199. doi: 10.1016/j.bioactmat.2023.03.024

Fig. 4.

Fig. 4

In vitro osteogenic assay of BLBs mineralized at different times. (A) The representative live/dead images of BMSCs cultured on BLBs at day 1, 4 and 7. (B) F‐actin cytoskeleton and nuclei of BMSCs cultured on BLBs at day 1. (C) The CCK-8 results of BMSCs proliferation at day 3, 7 and 14. (D) ALP activity of BMSCs at day 7 and 14. (E) OCN production of BMSCs at day 14 and 21. (F) Osteogenic gene expression results of BMSCs cultured on BLBs at day 14. The asterisk on top of the bar indicated a statistically significant difference between groups (p < 0.05).