Fig. 2.

The expression levels of Flu mRNA produced by VSW-3 or T7 RNAP in mice. (A) Schematic diagram of Flu mRNA-LNP preparation. (B) Size distribution of Flu mRNA-LNP particles in VSW-3 group and T7 group. (C) Flu mRNA-LNP prepared by VSW-3 or T7 were injected into mice via tail vein. Animals in mock group were injected with normal saline. Six hours after injection, the mice were anesthetized for imaging, and regions of interest (ROI) were determined by amiview software. (D) The Luciferase intensity of the VSW-3 and the T7 group were compared. Data are shown as mean ± SD. Unpaired t-test was used. ∗∗p < 0.01, n = 5.