Fig. 1.

GTS-21 promotesHIV-1transcription in CD4⁺T cells. ACH2 cells were treated with GTS-21 at different concentration for 48 h, (A) the expression level of gag mRNA was detected by RT-qPCR, (B) and specific primers were used to quantify the initiation and elongation of HIV-1 transcription. (C, D) Jurkat T cells were acutely infected with pseudovirus HIV-Luc/NL4-3 (5 ng p24) for 24 h and treated with GTS-21 for 48 h, gag mRNA and the initiation and elongation of HIV-1 transcription were detected. Result is one representative from three independent repeats. Data are presented as mean ​± ​SD. ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001 and ∗∗∗∗P ​< ​0.0001 denote significant difference.