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. 2022 Sep 29;1(5):100057. doi: 10.1016/j.cellin.2022.100057

Fig. 8.

Fig. 8

Trim25 mediated P degradation through autophagy. (A) 293T cells were co-transfected with FLAG-Trim25 and HA-CVS-P for 48 h and treated with 1 μM CQ 24 h before cell lysis. The cell lysates were analyzed by Western blotting. (B) 293T cells were co-transfected with FLAG-Trim25 and HA-CVS-P for 48 h and treated with 1 μM CQ 24 h before cell lysis; cell lysates were analyzed by Western blotting. (C) 293T cells were co-transfected with FLAG-Trim25 and HA-P-72m for 48 h and treated with 1 μM CQ 24 h before cell lysis; cell lysates were analyzed by Western blotting. (D) 293T cells were co-transfected with mCherry-GFP-LC3 and HA-CVS-P, or FLAG-Trim25, or HA-P-72m for 36 h and then analyzed for the co-localization of mCherry with GFP. The white arrow indicates the fluorescence reduction of GFP-LC3. Scale bar, 10 μm. Data are presented as mean ± SD, n = 3. Western blotting data are representative of at least three independent experiments.