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Effect of substituting lysine and P63 residues of HUα2 on DNA binding.
Binding of purified wild-type HUα2 or its variants harboring indicated amino acid substitutions to a 6-carboxy fluorescein labeled linear DNA or cruciform DNA. Each data point on Y-axis represents a change in milipolarization (mP) units at indicated protein concentrations in fluorescence polarizations assays. Kd (nM) estimated by fitting binding curves to a Hill equation are given in the table. ND = not determined.
